Device

Part:BBa_K1819008:Design

Designed by: Laís Ribovski   Group: iGEM15_Brasil-USP   (2015-09-16)


Promoter test circuit to analyze the kill switch efficiency


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2437
    Illegal AgeI site found at 2549
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

Two different fluorescent protein, GFP and RFP, were chosen to detect expression variation considering their emission and excitation wavelength to avoid spectra superposition.


Source

This part results from the assembly of BBa_R0010, BBa_B0034, BBa_E0040, BBa_P0440, BBa_R0040 and BBa_I13507.

References